Real-Time PCR

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Real-Time PCR (qPCR) miRNA QRT-PCR 

Agilent - miRNA QRT-PCR Detection Kit

Agilent - miRNA QRT-PCR Detection Kit

Detect mature miRNA from as little as 15 ng of total RNA

The miRNA QRT-PCR and miRNA QRT-PCR Master Mix Detection Kits detect mature miRNA from as little as 15 ng of total RNA input on various sample types. The detection kits also differentiate between miRNAs that differ by as few as a single nucleotide. 

  • Detects mature miRNA

  • Differentiates miRNA that differ by a single nucleotide

  • Sensitive detection down to 10 copies

  • Highly accurate results in 3 hours

  • miRNA Specific Forward Primers

  • miRNA Primer Design Guidelines


Real-Time PCR (qPCR) cDNA Synthesis Kits

Agilent cDNA Synthesis Kit

Agilent - cDNA Synthesis Kit

cDNA Synthesis

Using the AffinityScript cDNA Synthesis Kit you are ensured efficient conversion of RNA to cDNA. It is fully optimized for real-time quantitative PCR (qPCR) applications and kit utilizes qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

  • Optimized for real-time quantitative PCR (qPCR) applications
  • Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA
  • Creates up to 12 kb cDNA in 25 minutes or less
  • Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format


Agilent -  Brilliant III qPCR and Brilliant HRM Master Mixes

Agilent -  Brilliant III qPCR and Brilliant HRM Master Mixes

Brilliant SNPtyping with Agilent Master Mixes

Brilliant III ultrafast probe Master Mixes provide specificity by utilizing a novel faster-activating hot start method. The hot start method minimizes the formation of primer-dimers and other off-target reactions. These reagents are robust and reproducible across fast cycling real-time platforms.

Brilliant III QPCR Ultra-Fast Probe Master Mix

  • Extremely fast runs while maintaining sensitivity and great resistance to qPCR inhibitors
  • Optimized protocols for most qPCR instruments

Brilliant HRM Ultra-Fast Loci Master Mix

  • Platform-independent mix for high-resolution melt (HRM) analysis
  • Mutant Fast-Start Taq polymerase, optimized MgCl2 and dNTPs concentrations

  • EvaGreen, release-on-demand dye for fast results for the most difficult genotypes

Multiplex Gene Expression Assay

eBioscience - QuantiGene™ Plex

eBioscience - QuantiGene™ Plex

Multiplexed gene expression quantitation

Accurate and precise RNA quantitation of up to 80 genes in a single well.

  • Multiplex and customizable RNA or DNA measurement using a Luminex® instrument
  • No need for RNA purification or target amplification - just lyse and run
  • Works with difficult sample types, including FFPE, and directly with blood

Preamp and Reverse Transcription Master Mixes

Fluidigm - Master Mixes Preamp and Reverse Transcription Master Mixes

Fluidigm - Master Mixes

Preamp and Reverse Transcription Master Mixes

Fluidigm delivers optimized Preamp and Reverse Transcription Master Mixes for the Biomark and Biomark HD platforms. Preamplification allows for multiplex amplification of up to 96 targets. A pool of primers is prepared from the same gene expression assays to be used for qPCR. By using the real-time qPCR assays in the preamplification reaction, only the targets of interest are amplified. A limited number of cycles is used and the cDNA is amplified without significant bias.

  • Optimized for small volumes
  • Optimized for TaqMan Gene Expression Assays

Integrated Fluidic Circuits (IFCs)

Fluidigm - Biomark HD IFCs

Fluidigm - Biomark HD IFCs

Make up to 9.216 datapoints in one run

Enhance your productivity with high-performance solutions for robust processing of samples in nanoscale reactions, enabling quick and reliable gene expression, copy count, and digital PCR. The revolutionary integrated fluidic circuits (IFCs) enable automated molecular biology that significantly minimizes pipetting steps, hands-on time, and reagent consumption while maximizing efficiency, cost savings, and robust data production.

  • Find the format that covers your project from 12-192 samples combined with 12-192 assays
  • Sample.Assays:
  • 192.24
  • 96.96
  • 48.48
  • 24.192
  • 12.12*6